细粒棘球蚴原头蚴包囊和原头蚴成虫抑制差减杂交文库中差异表达基因的筛选及分析

doi:10.11843/j.issn.0366-6964.2015.07.019

Abstract

Abstract:

According to the bidirectional development of the protoscolex (PSC) of Echinococcus granulosus，the study was conducted to screen the specifical genes of E.granulosus PSC development between a larval cyst and adult worm.The subtractive PCR products of the SSH library of E.granulosus PSC-adult (AW) and E.granulosus PSC-cyst (CW) were connected to the pGEM-T easy vector，and were cloned and sequenced.Totally，280 and 200 clones were obtained from PSC-CW and PSC-AM libraries，respectively，with the length of fragments 200 to 1000 bp.The clones were sequenced.Sequence alignment analysis showed that these sequences belong to 16 and 10 gene clusters representing 16 and 10 unique genes from PSC-CM and PSC-AW libraries respectively.As the genes are specifically or differentially expressed during the development of PSC to either adult worm or cyst，they are important for the parasite development and differentiation，thus can be candidates for drug and vaccine development.

CLC Number:

S852.734

ZHAO Li，SHI Bao-xin，LI Jun，ZHANG Zhuang-zhi，MA Zheng-hai，ZHANG Xu，XUE Jing，JIN Ying-hong，ZHANG Wen-bao. The Screening and Analysis of Differentially Expressed Genes in Suppression Subtractive Hybridization Library with either Cyst (CW) or Adult Worm (AM) Development from Echinococcus granulosus Larval Protoscolex (PSC)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1215-1223.

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The Screening and Analysis of Differentially Expressed Genes in Suppression Subtractive Hybridization Library with either Cyst (CW) or Adult Worm (AM) Development from Echinococcus granulosus Larval Protoscolex (PSC)

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